Phytoplasma Identifi cation in Opuntia monacantha var. variegata from a Private Collection of Russia

Bogoutdinov D.Z., Girsova N.V., Kastalyeva T.B.

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2021. T. 126. Vyp. 5.

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2021. T. 126. Vyp. 5.

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Bogoutdinov D.Z., Girsova N.V., Kastalyeva T.B.

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Opuntia monacantha var. variegata Haworth., phytoplasma, 16SrII-C, cactus witches’-broom phytoplasma, PCR and RFLP analysis

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Bogoutdinov D.Z., Girsova N.V., Kastalyeva T.B. , Phytoplasma Identifi cation in Opuntia monacantha var. variegata from a Private Collection of Russia // Byul. MOIP. Otd. biol. 2021. T. 126. Vyp. 5. S. 23-30

Phytoplasma Identifi cation in Opuntia monacantha var. variegata from a Private Collection of Russia

Opuntia monacantha commonly known as prickly pear cactus from a private collection of the Samara region, had an unusual shape and color of cladodes, which suggested the presence of infection with phytoplasma that was confirmed using polymerase chain reaction (PCR) in 2015. However, the taxonomic belonging of phytoplasma to the group was not determined due to the low concentration of the pathogen. The prickly pears analyzed were rooted and continued to grow in the greenhouse for five years. As a result, numerous small cladodes were formed on each of the main stems. PCR and analysis of restriction fragment length polymorphism revealed a high degree of infection of prickly pear with witch’s broom peanut phytoplasma ‒ 16SrII group. Restriction patterns obtained after PCR product digestions with AluI, Bsh1236I (BstU1), HinfI, HhaI, HpaII, RsaI, TaqI and TruI1 (MseI) endonucleases allow phytoplasma to be identified as belonging to the 16SrII-С subgroup.

References

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Bertaccini A., Franova J., Botti S., and Tabanelli D. Molecular characterization of phytoplasmas in lilies with fasciation in the Czech Republic // FEMS Microbiology Letters, 2005. Vol. 249. P. 79–85.
Cai H, Chen H.R., Li F., Kong B.H. First report of a phytoplasma associated with cactus witches’-broom in Yunnan (China) // Plant Pathology. 2002. Vol. 51. P. 394.
Cai H., Chen H.R., Kong B.H., Yang G.H., Liu T. First report of a phytoplasma associated with Christmas cactus witches’ broom // Plant Pathology. 2007. Vol. 56. P. 346.
Cai H., Wei W., Davis R.E., Chen H., Zhao Y. Genetic diversity among phytoplasmas infecting Opuntia species: virtual RFLP analysis identifies new subgroups in the peanut witches’-broom phytoplasma group // International Journal of Systematic and Evolutionary Microbiology. 2008. Vol. 58. P. 1448–1457.
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Marathi H., Owati A., Madata C.S., Joosten M., Beed F., Kumar P.L. First Report of 16SrII-C Subgroup Phytoplasma Causing Phyllody and Witches’– broom Disease in Soybean in Tanzania // Plant Disease. 2015. Vol. 99(6). P. 886 (DOI: 10.1094/PDIS-11-14-1225-PDN).
Omar A.F., Dewir Y.H., El-Mahrouk M.E. Molecular identification of phytoplasmas in fasciated cacti and succulent species and associated hormonal perturbation // Journal of Plant Interactions. 2014. Vol.9. P. 632–639.
Padilla K.A., Parra-Cota F., Ochoa-Sánchez J.C. Phytoplasmas associated to diseases of ornamental cacti in Mexico // Journal of Biological Sciences. 2009. Vol. 9. P. 268–271.
Salehi M., Esmailzadeh Hosseini S.A., Salehi E., Bertaccini A. Molecular and biological characterization of a 16SrII phytoplasma associated with carrot witches’ broom in Iran. // J. Plant Pathology. 2016. Vol. 98. P. 83–90 (https://www.jstor.org/stable/24892625).
Tessitori M., Masenga V., Marzachì C. First report of a phytoplasma associated with abnormal proliferation of cladodes in cactus pear (Opuntia ficus-indica) in Italy // J. Plant Pathology. 2006. Vol. 55. P. 292.
Yadav A., Thorat V., Bhale U., Shouche Y. Association of 16SrII-C and 16SrII-D subgroup phytoplasma strains with witches’broom disease of Parthenium hysterophorus and insect vector Orosius albicinctus in India //Australasian Plant Disease Notes. 2015. Vol. 10. P. 31 (DOI:10.1007/s13314-015-0181-2).
Zak L.F., Yáñz-Morales M.J., Alanis-Martínez I., González-Pérez E. New hosts of 16SrI phytoplasma group associated with edible Opuntia ficus-indica crop and its pests in Mexico // African Journal of Microbiology Research. 2011. Vol. 5. P. 910–918.

Список литературы

Matyashova G.N. Razrabotka i sovershenstvovanie metodov diagnostiki fitoplazm ‒ vozbuditelei boleznei plodovykh i yagodnykh kul’tur: avtoref. dis. … kand. biol. nauk. M., 2017. 22 s.
Akhtar K.P., Dickinson M., Asghar M.J., Abbas G., Sarwar N. Association of 16SrII-C phytoplasma with lentil phyllody disease in Pakistan // Tropical Plant Pathology. 2016. Vol. 41. P.198–202 (DOI 10.1007/s40858-016-0087-3).
Al-Saady N.A., Al Subhi A.M., Al-Nabhani A., Khan A.J. First report of a group 16 SrII phytoplasma infecting chickpea in Oman // Plant Disease. 2006. Vol. 90. P. 973 (DOI: 10.1094/PD-90-0973C).
Al-Subhi A., Hogenhout S.A., Al-Yahyai R.A., Al-Sadi A.M. Classification of a new phytoplasmas subgroup 16SrII-W associated with Crotalaria witches’ broom diseases in Oman based on multigene sequence analysis // BMC Microbiol. 2017. Vol. 17. P. 221 (DOI: 10.1186/s12866-017-1130-3).
Bertaccini A., Calari A., Felker P. Developing a method for phytoplasma identification in cactus pear samples from California // Bulletin of Insectology. 2007. Vol. 60. P. 257–258.
Bertaccini A., Franova J., Botti S., and Tabanelli D. Molecular characterization of phytoplasmas in lilies with fasciation in the Czech Republic // FEMS Microbiology Letters, 2005. Vol. 249. P. 79–85.
Cai H, Chen H.R., Li F., Kong B.H. First report of a phytoplasma associated with cactus witches’-broom in Yunnan (China) // Plant Pathology. 2002. Vol. 51. P. 394.
Cai H., Chen H.R., Kong B.H., Yang G.H., Liu T. First report of a phytoplasma associated with Christmas cactus witches’ broom // Plant Pathology. 2007. Vol. 56. P. 346.
Cai H., Wei W., Davis R.E., Chen H., Zhao Y. Genetic diversity among phytoplasmas infecting Opuntia species: virtual RFLP analysis identifies new subgroups in the peanut witches’-broom phytoplasma group // International Journal of Systematic and Evolutionary Microbiology. 2008. Vol. 58. P. 1448–1457.
Casper R., Lesemann D., Bartels R. Mycoplasma-like bodies and viruses in Opuntia tuna with witches’-broom disease // Plant Disease Reporter. 1970. Vol. 54. N 50. P. 851–853.
Choueiri E., Massad R., Jreijiri F., Danet J.L., Salar P., Bové J.M., Foissac X. First report of a 16SrII group phytoplasma associated with shoot proliferation of a cactus (Opuntia monacantha) in Lebanon // Plant Disease. 2005. Vol.89. P.1129 (http://dx.doi.org/10.1094/PD-89-1129B).
Dewir Y.H. Cacti and succulent plant species as phytoplasma hosts: A review // Phytopathogenic Mollicutes. 2016. Vol. 6 (1). P. 1–9 (DOI: 10.5958/2249-4677.2016.00001.3).
Dewir Y.H., El-Mahrouk M.E., Omar A.F. Molecular characterization of 16SrII phytoplasma group and associated down-regulation of ABA and IAA in Opuntia caracasana // Phytopathogenic Mollicutes. 2015. Vol. 5. P. 100–106.
Girsova N.V., Bottner-Parker K.D., Bogoutdinov D.Z., Meshkov Y.I., Mozhaeva K.A., Kastalyeva T.B., Lee I.-M. Diverse phytoplasmas associated with potato stolbur and other related potato diseases in Russia // Eur. J. Plant Pathol. 2016. Vol. 145. P. 139–153 (DOI: 10.1007/s10658-015-0824-3).
Granata G., Paltrinieri S., Botti S., Bertaccini A. Aetiology of Opuntia ficus-indica malformations and stunting disease // Annals of Applied Biology. 2006. Vol. 149. P. 317–325.
Hernández-Pérez, R, Noa-Carrazana, J C, Gaspar, R, Mata, P and Flores-Estévez, N. First report of symptoms associated to a phytoplasma affecting Nopal (Opuntia ficus-indica Mill) in the “Pyramids” Indian, State of Mexico // Journal of General and Molecular Virology. 2009. Vol. 1(4). P. 46–47.
Leyva-Lopez N.E., Aguilar-Rojas O.I,, Leal-Klevezas D.S., Martinez-Soriano J.P. Presence of phytoplasmas in Mexican cacti // Phytopathology. 1999. Vol. 89. S. 45.
Marathi H., Owati A., Madata C.S., Joosten M., Beed F., Kumar P.L. First Report of 16SrII-C Subgroup Phytoplasma Causing Phyllody and Witches’– broom Disease in Soybean in Tanzania // Plant Disease. 2015. Vol. 99(6). P. 886 (DOI: 10.1094/PDIS-11-14-1225-PDN).
Omar A.F., Dewir Y.H., El-Mahrouk M.E. Molecular identification of phytoplasmas in fasciated cacti and succulent species and associated hormonal perturbation // Journal of Plant Interactions. 2014. Vol.9. P. 632–639.
Padilla K.A., Parra-Cota F., Ochoa-Sánchez J.C. Phytoplasmas associated to diseases of ornamental cacti in Mexico // Journal of Biological Sciences. 2009. Vol. 9. P. 268–271.
Salehi M., Esmailzadeh Hosseini S.A., Salehi E., Bertaccini A. Molecular and biological characterization of a 16SrII phytoplasma associated with carrot witches’ broom in Iran. // J. Plant Pathology. 2016. Vol. 98. P. 83–90 (https://www.jstor.org/stable/24892625).
Tessitori M., Masenga V., Marzachì C. First report of a phytoplasma associated with abnormal proliferation of cladodes in cactus pear (Opuntia ficus-indica) in Italy // J. Plant Pathology. 2006. Vol. 55. P. 292.
Yadav A., Thorat V., Bhale U., Shouche Y. Association of 16SrII-C and 16SrII-D subgroup phytoplasma strains with witches’broom disease of Parthenium hysterophorus and insect vector Orosius albicinctus in India //Australasian Plant Disease Notes. 2015. Vol. 10. P. 31 (DOI:10.1007/s13314-015-0181-2).
Zak L.F., Yáñz-Morales M.J., Alanis-Martínez I., González-Pérez E. New hosts of 16SrI phytoplasma group associated with edible Opuntia ficus-indica crop and its pests in Mexico // African Journal of Microbiology Research. 2011. Vol. 5. P. 910–918.