Interaction of microalgae in a mixed culture under toxic exposure

Ipatova V.I.

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2025. T. 130. Vyp. 2.

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2025. T. 130. Vyp. 2.

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10.55959/MSU0027-1403-BB-2025-130-2-18-28

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Ipatova V.I.

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mixed culture, Scenedesmus quadricauda, Monoraphidium arcuatum, biotic interaction, bioassay, sodium molybdate

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Ipatova V.I. , Interaction of microalgae in a mixed culture under toxic exposure // Byul. MOIP. Otd. biol. 2025. T. 130. Vyp. 2. S. 18-28

Interaction of microalgae in a mixed culture under toxic exposure

The interaction between two species of microalgae Scenedesmus quadricauda (Turp.) Breb. and Monoraphidium arcuatum (Korsch.) Hind. was studied in a mixed culture at different levels of exposure to molybdenum in the form of sodium molybdate – low, medium and high concentrations of the toxicant from 20 to 400 mg/Mo/l in chronic experiments lasting 21 days. The most integral test parameter in bioassay using mixed cultures of microalgae is the number of cells, which makes it possible to take into account the ratio of individual species. A two-species toxicity test using freshwater microalgae in culture showed that the growth rate of the laboratory population of each species was different from those in tests on individual species, both in control variants (without toxicants) and during intoxication. It has been shown that the sensitivity of each species in monoculture is higher than in mixed culture in both acute and chronic experiments. In a mixed culture, at low level of exposure to sodium molybdate (20 mg Mo/l), M. arcuatum is more sensitive to competitive displacement by S. quadricauda, and at high levels of exposure (100–400 mg Mo/l), M. arcuatum becomes more resistant to competitive displacement over a long period of time. This effect of the toxicant on the two species test system should be taken into account for a more adequate assessment of the toxicity of substances to microalgae. The findings suggest that single-species bioassays in standard biological assays may underestimate the toxicity of metals in natural waters.

References

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Passarge J., Hol S., Escher M., Huisman J. Competition for nutrients and light: stable coexitence, alternative stable states, or competitive exclusion? // Ecol. Monogr. 2006. Vol. 76. P. 57-72.
Picone M., Bergamin M., Losso C., Delaney E., Novelli A., Ghirardini A. Assessment of sediment toxicity in the Lagoon of Venice (Italy) using a multi-species set of bioassays // Ecotoxicol. Environ. Saf. 2016. Vol. 123. P. 32–44.
Stauber J.L. Toxicity testing using marine and freshwater unicellular algae. Australas. J. Ecotoxicol. 1995. Vol. 1. P. 15-24.
Swartzman G.L., Taub F.B., Meador J., Huang C., Kindig A. Modeling the effect of algal biomass on multispecies aquatic microcosms response to copper toxicity // Aquat. Toxicol. 1990. Vol. 17. P. 93–118.
Wang C., Wang Z., Zhang Y., Su R. Interspecies interactions reverse the hazard of antibiotics exposure: a plankton community study on responses to ciprofloxacin hydrochloride // Sci. Rep. 2017. Vol. 7: 2373.
Yu Y., Kong F., Wang M., Qian L., Shi X. Determination of short-term copper toxicity in a multispecies microalgal population using flow cytometry // Ecotoxicol. Environ. Saf. 2007. Vol. 66. P. 49–56.

Список литературы

Metodika opredeleniya toksichnosti vod, vodnykh vytyazhek iz pochv, osadkov stochnykh vod i otkhodov po izmeneniyu urovnya fluorestsentsii khlorofilla i chislennosti kletok vodoroslei. M., 2007. 48 s. (in Russ.)
Metodicheskie ukazaniya po razrabotke normativov kachestva vody vodnykh ob»ektov rybokhozyaistvennogo znacheniya, v tom chisle normativov predel’no dopustimykh kontsentratsii vrednykh veshchestv v vodakh vodnykh ob»ektov rybokhozyaistvennogo znacheniya. Pod red. O.F. Filenko, S.A. Sokolovoi. M., 2011. 165 s. (in Russ.)
Metody ispytanii khimicheskoi produktsii, predstavlyayushchei opasnost’ dlya okruzhayushchei sredy. Ispytanie vodoroslei i tsianobakterii na zaderzhku rosta. GOST 32293-2013 (OECD, Test No. 201, IDT). M., 2015. 24 s. (in Russ.)
Miheev M.A., Ipatova V.I., Spirkina N.E. Biotic Interactions between Two Species of Microalgae in Mixed Culture // Moscow University Biological Sciences Bulletin. 2018. T. 73. № 2. S. 63-68
Spirkina N.E., Dmitrieva A.G., Filenko O.F., Ipatova V.I. Vliyanie bikhromata kaliya na razvitie i strukturu populyatsii odnokletochnoi khlorokokkovoi vodorosli Monoraphidium arcuatum (Korsch.) Hind. // Ekologicheskie sistemy i pribory. 2014a. № 4. S. 36-42 (in Russ.)
Spirkina N.E., Ipatova V.I., Dmitrieva A.G., Filenko O.F. Comparative Growth of Microalgae Cultures of Species Monoraphidium arcuatum (Korsch.) Hind. and Scenedesmus quadricauda (Turp.) Bréb. // Byul. MOIP. Otdel biol. 2014. T. 119. № 2. S. 64–69 (in Russ.)
Terekhova V.A., Voronina L.P., Gershkovich D.M., Ipatova V.I., Isakova E.F., Kotelevtsev S.V., Poputnikova T.O., Rakhleeva A.A., Samoilova T.A., Filenko O.F. Biotest-sistemy dlya zadach ekologicheskogo kontrolya: Metodicheskie reko mendatsii po prakticheskomu ispol’zovaniyu standartizovannykh test-kul’tur. M., 2014. 48 s. (in Russ.)
Terekhova V.A., Gershkovich D.M., Gladkova M.M., Ipatova V.I., Isakova E.F., Nikolaeva O.V., Rakhleeva A.A., Fedoseeva E.V. Biotestirovanie v ekologicheskom kontrole / Pod red. V.A. Terekhovoi. M.: GEOS, 2017. 70 s. (in Russ.)
Filenko O.F., Isakova E.F., Gershkovich D.M., Ipatova V.I., Dmitrieva A.G. Biotestirovanie kachestva sredy s ispol’zovaniem gidrobiontov. Razdel bol’nogo praktikuma po gidrobiologii: Uchebno-metodicheskoe posobie. M., 2015. 44 s. (in Russ.)
Bautista-Chamizo E., Sendra M., De Orte M.R., Riba I. Comparative effects of seawater acidification on microalgae: Single and multispecies toxicity tests // Sci. Tot. Environ. 2019. Vol. 649. P. 224-232.
Bautista-Chamizo E., Sendra M., De Orte M.R., Riba I. Comparative effects of seawater acidification on microalgae: Single and multispecies toxicity tests // Sci. Tot. Environ. 2019. Vol. 649. P. 224-232.
Cheloni G., Gagnaux V., Slaveykova V.I. Species-species interactions modulate copper toxicity under different visible light conditions // Ecotoxicol. Environ. Saf. 2019. Vol. 170. P. 771–777.
Clements W.H., Rohr J.R. Community responses to contaminants: using basic ecological principles to predict ecotoxicological effects // Environ. Toxicol. Chem. 2009. Vol. 28. P. 1789–1800.
Debenest T., Petit A.N., Gagné F., Kohli M., Nguyen N., Blaise C. Comparative toxicity of a brominated flame retardant (tetrabromobisphenol A) on microalgae with single and multi-species bioassays // Chemosphere 2011. Vol. 85. P. 50–55.
De Morais P., Stoichev T., Basto M.C.P., Ramos V., Vasconcelos V.M., Vasconcelos M.T.S.D. Pentachlorophenol toxicity to a mixture of Microcystis aeruginosa and Chlorella vulgaris cultures // Aquat. Toxicol. 2014. Vol. 150. P. 159–164.
De Laender F., De Schamphelaere K.A.C., Vanrolleghem P.A., Janssen C.R. Comparing ecotoxicological effect concentrations of chemicals established in multi-species vs. single-species toxicity test systems // Ecotoxicol. Environ. Saf. 2009. Vol. 72. P. 310–315.
Fawaz E.G., Kamareddine L.A., Salam D.A. Effect of algal surface area and species interactions in toxicity testing bioassays // Ecotoxicol. Environ. Saf. 2019. Vol. 174. P. 584–591.
Franklin N.M., Stauber J.L., Lim R.P. Development of multispecies algal bioassays using flow cytometry // Environ. Toxicol. Chem. 2004. Vol. 23. P. 1452-1462.
Leflaive J., Ten-Hage L. Algal and cyanobacterial secondary metabolites in freshwaters: a comparison of allelopathic compounds and toxins // Freshw. Biol. 2007. Vol. 52. P. 199-214.
Passarge J., Hol S., Escher M., Huisman J. Competition for nutrients and light: stable coexitence, alternative stable states, or competitive exclusion? // Ecol. Monogr. 2006. Vol. 76. P. 57-72.
Picone M., Bergamin M., Losso C., Delaney E., Novelli A., Ghirardini A. Assessment of sediment toxicity in the Lagoon of Venice (Italy) using a multi-species set of bioassays // Ecotoxicol. Environ. Saf. 2016. Vol. 123. P. 32–44.
Stauber J.L. Toxicity testing using marine and freshwater unicellular algae. Australas. J. Ecotoxicol. 1995. Vol. 1. P. 15-24.
Swartzman G.L., Taub F.B., Meador J., Huang C., Kindig A. Modeling the effect of algal biomass on multispecies aquatic microcosms response to copper toxicity // Aquat. Toxicol. 1990. Vol. 17. P. 93–118.
Wang C., Wang Z., Zhang Y., Su R. Interspecies interactions reverse the hazard of antibiotics exposure: a plankton community study on responses to ciprofloxacin hydrochloride // Sci. Rep. 2017. Vol. 7: 2373.
Yu Y., Kong F., Wang M., Qian L., Shi X. Determination of short-term copper toxicity in a multispecies microalgal population using flow cytometry // Ecotoxicol. Environ. Saf. 2007. Vol. 66. P. 49–56.