Бюллетень Московского общества испытателей природы. Отдел биологический
Bulletin of Moscow Society of Naturalists.<br /> Biological Series
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2023. T. 128. Vyp. 5.
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2023. T. 128. Vyp. 5.

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DOI

10.55959/MSU0027-1403-BB-2023-128-5-72-84

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Authors:

Kovtonyuk N.K., Yudanova S.S., Nuzhdina N.S.

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Keywords:

voucher samples, herbarium collections, molecular markers, ITS, ISSR, vascular plants, A.P. Khokhryakov, Primula sect. Aleuritia

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Kovtonyuk N.K., Yudanova S.S., Nuzhdina N.S., Taxonomic status and genetic variation in Primula mazurenkoae (Primulaceae): evidence from ISSR-analysis and ITS sequencing // Byul. MOIP. Otd. biol. 2023. T. 128. Vyp. 5. S. 72-84

Taxonomic status and genetic variation in Primula mazurenkoae (Primulaceae): evidence from ISSR-analysis and ITS sequencing

The study aiming to clarify the taxonomic status of Primula mazurenkoae (Aleuritia section, Primulaceae) and determining the level of its genetic divergence in relation to the morphologically close P. farinosa was carried out. To study the genetic relationships between the closely related species, ISSR analysis was performed using five primers; 23 nucleotide sequences of nrITS region were obtained; 20 of them were revealed in the present study. Genetic analyses revealed a high level of variability in natural populations of P. mazurenkoae. Here we can conclude that the level of morphological similarity between P. mazurenkoae and P. farinosa correlates with the results of ISSR analysis and ITS nucleotide data obtained in the study.

References

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  • Kovtonyuk N.K. Semeistvo Primulaceae Batsch ex Borkh. // Konspekt fl ory Aziatskoi Rossii. Sosudistye rasteniya. Novosibirsk, 2012. S. 130–137.
  • Kovtonyuk N.K. Primula bukukunica – novyi vid roda Primula (Primulaceae) // Botanicheskii zhurnal. 2009. T. 94. № 12. S. 1835–1841.
  • Kovtonyuk N.K. (red.) Tsifrovoi gerbarii TsSBS SO RAN: Elektronnyi resurs. Novosibirsk: TsSBS SO RAN. 2023. Rezhim dostupa: http://herb.csbg.nsc.ru:8081 (data obrashcheniya 06.03.2023).
  • Probatova N.S. Pervotsvet, ili Primula – Primula L. // Sosudistye rasteniya sovetskogo Dal’nego Vostoka. L., 1987. T. 2. S. 139–151.
  • Seregin A. P. (red.) Tsifrovoi gerbarii MGU: Elektronnyi resurs. M.: MGU, 2023. Rezhim dostupa: https://plant.depo.msu.ru/ (data obrashcheniya 06.03.2023).
  • Suprun N.A., Shantser I.A. Geneticheskaya izmenchivost’ vidov rodstva Hedysarum grandiflorum Pall. (Fabaceae) po dannym ISSR markirovaniya // Byulleten’ Glavnogo botanicheskogo sada. 2012. T. 198. Vyp. 4. S. 41–48.
  • Fedorov An. A. Pervotsvet – Primula L. // Flora SSSR. M.; L., 1952. T. 18. S. 111–202.
  • Khokhryakov A.P. Flora Magadanskoi oblasti. M., 1985. 397s.
  • Khokhryakov A.P. Desyat’ novykh vidov i podvidov tsvetkovykh rastenii iz severo-vostochnoi Azii // Byull. MOIP Otd. biol. 1984. T. 89. Vyp. 4. S. 107–108.
  • Baasanmunkh S., Kovtonyuk N.K., Oyuntsetseg B., Tsegmed Z., Han I.V., Choi H.J. Diversity and distribution of the genus Primula L. (Primulaceae) in Mongolia // Journal of Asia-Pacific Biodiversity. 2020. Vol. 13. N 4. P. 687–700.
  • Conti E., Suring E., Boyd D., Jorgensen J., Grant J., Kelso S. Phylogenetic relatioships and character evolution in Primula L.: the usefulness of ITS sequence data // Plant Biosystems. 2000. Vol. 134. N 3. P. 385–392.
  • Crema S., Cristofolini G., Rossi M., Conte L. High genetic diversity detected in the endemic Primula apennina Widmer (Primulaceae) using ISSR fi ngerprinting // Plant Systematics and Evolution. 2009. Vol. 280. P. 29–36.
  • GBIF: The Global Biodiversity Information Facility. 2023. Available from: https://www.gbif.org. Last accessed 17.04.2023.
  • Guggisberg A., Mansion G., Kelso S., Conti E. Evolution of biogeographic patterns, ploidy levels, and breeding systems in a diploid–polyploid species complex of Primula // New Phytologist. 2006. Vol. 171. N 3. P. 617–632.
  • Hu C.M., Kelso S. Primulaceae // Flora of China. Vol. 15. (Myrsinaceae through Loganiaceae). Beijing, St. Louis. 1996. P. 39–189.
  • Hulten E. Flora of Alaska and Yukon // Acta Universitatis Lundensis. 1948. Vol. 44, N 1. P. 1268.
  • Kovtonyuk N.K., Gontcharov A.A. Phylogenetic relationships in the genus Primula (Primulaceae) inferred from the ITS region sequences of nuclear rDNA // Russian Journal of Genetics. 2009. Vol. 45. N 6. P. 663–670.
  • Martins L., Oberprieler C., Hellwig F. A phylogenetic analysis of Primulaceae s.l. based on internal transcribed spacer (ITS) DNA sequence data // Plant Systematics and Evolution. 2003. Vol. 237. P. 75–85.
  • Mast A.R., Kelso S., Richards A.J., Lang D.J., Feller D.M.S., Conti E. Phylogenetic relationships in Primula L. and related genera (Primulaceae) based on noncoding chloroplast DNA // International Journal of Plant Sciences. 2001. Vol. 162. P. 1381–1400.
  • Nan P., Shi S., Peng S., Tian Ch., Zhong Y. Genetic diversity in Primula obconica (Primulaceae) from Central and South-west China as revealed by ISSR Markers // Annals of Botany. 2003. Vol. 91. P. 329–333.
  • Nei M., Li W.H. Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases // PNAS. 1979. Vol. 76. N 10. P. 5269–5273.
  • Nuzhdina N.S., Kovtonyuk N.K. Genotyping NS and NSK herbaria specimens of Hedysarum (Fabaceae) using DNA sequencing // Botanica Pacifica. A journal of plant science and conservation. 2022. Т. 11. N 1. P. 176–180.
  • POWO. Plants of the World Online. 2023. Facilitated by the Royal Botanic Gardens, Kew. Published on the Internet; http://www.plantsoftheworldonline.org [Retrieved 06 March 2023].
  • Richards J. Primula. Timber Press, Portland, Oregon, USA. 346 p.
  • Schmidt–Lebuhn, A.N., Vos J.M., Keller B., Conti E. 2012. Phylogenetic analysis of Primula section Primula reveals rampant non–monophyly among morphologically distinct species // Molecular Phylogenetics and Evoution. 2012. Vol. 65. N 1. P. 23–34.
  • Tamura K., Stecher G., Ku mar S. MEGA11: Molecular Evolutionary Genetics Analysis Version 11 // Molecular Biology and Evolution. 2001. Vol. 38. N 7. P. 3022–3027.
  • Theodoridis S., Nogues Bravo D., Conti E. The role of cryptic diversity and its environmental correlates in global conservation status assessments: Insights from the threatened bird’s eye primrose (Primula farinosa L.) // Diversity and Distributions. 2019. Vol. 25. P. 1457–1471.
  • Van de Peer Y., De Wachter R. TREECON for Windows: A software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment // Computer Applications in the Biosciences. 1994. Vol. 10. P. 569–570.
  • Wolfe A.D. ISSR techniques for evolutionary biology // Methods of Enzymology. 2005. Vol. 395. P. 134–144.
  • Yan H.-F., Hao G., Hu C.-M., Ge X.-J. DNA barcoding in closely related species: A case study of Primula L. sect. Proliferae Pax (Primulaceae) in China // Journal of Systematics and Evolution. 2011. Vol. 49, N 3. P. 225–236.
  • Yudanova S.S., Plugatar S.A., Klimenko Z.K., Zykova V.K., Rubtsova O.L., Vasilyeva O.Yu. Genetic diversity analysis of Rose varieties from Grandifl ora group based on ISSR molecular markers // Northern Asia Plant Diversity. BIO Web of Conferences. 2021. Vol. 38. 00140. https://doi.org/10.1051/bioconf/20213800140
  • Zhang L.B., Kadereit J.W. Classifi cation of Primula sect. Auricula (Primulaceae) based on two molecular data sets (ITS, AFLPs), morphology and geographical distribution // Botanical Journal of the Linnean Society. 2004. Vol. 146. P. 1–26.

Список литературы

  • Bush E.A. Sem. 65. Primulaceae // Flora Sibiri i Dal’nego Vostoka. L., 1926. S. 7–81.
  • Kovtonyuk N.K. Semeistvo Primulaceae Batsch ex Borkh. // Konspekt fl ory Aziatskoi Rossii. Sosudistye rasteniya. Novosibirsk, 2012. S. 130–137.
  • Kovtonyuk N.K. Primula bukukunica – novyi vid roda Primula (Primulaceae) // Botanicheskii zhurnal. 2009. T. 94. № 12. S. 1835–1841.
  • Kovtonyuk N.K. (red.) Tsifrovoi gerbarii TsSBS SO RAN: Elektronnyi resurs. Novosibirsk: TsSBS SO RAN. 2023. Rezhim dostupa: http://herb.csbg.nsc.ru:8081 (data obrashcheniya 06.03.2023).
  • Probatova N.S. Pervotsvet, ili Primula – Primula L. // Sosudistye rasteniya sovetskogo Dal’nego Vostoka. L., 1987. T. 2. S. 139–151.
  • Seregin A. P. (red.) Tsifrovoi gerbarii MGU: Elektronnyi resurs. M.: MGU, 2023. Rezhim dostupa: https://plant.depo.msu.ru/ (data obrashcheniya 06.03.2023).
  • Suprun N.A., Shantser I.A. Geneticheskaya izmenchivost’ vidov rodstva Hedysarum grandiflorum Pall. (Fabaceae) po dannym ISSR markirovaniya // Byulleten’ Glavnogo botanicheskogo sada. 2012. T. 198. Vyp. 4. S. 41–48.
  • Fedorov An. A. Pervotsvet – Primula L. // Flora SSSR. M.; L., 1952. T. 18. S. 111–202.
  • Khokhryakov A.P. Flora Magadanskoi oblasti. M., 1985. 397s.
  • Khokhryakov A.P. Desyat’ novykh vidov i podvidov tsvetkovykh rastenii iz severo-vostochnoi Azii // Byull. MOIP Otd. biol. 1984. T. 89. Vyp. 4. S. 107–108.
  • Baasanmunkh S., Kovtonyuk N.K., Oyuntsetseg B., Tsegmed Z., Han I.V., Choi H.J. Diversity and distribution of the genus Primula L. (Primulaceae) in Mongolia // Journal of Asia-Pacific Biodiversity. 2020. Vol. 13. N 4. P. 687–700.
  • Conti E., Suring E., Boyd D., Jorgensen J., Grant J., Kelso S. Phylogenetic relatioships and character evolution in Primula L.: the usefulness of ITS sequence data // Plant Biosystems. 2000. Vol. 134. N 3. P. 385–392.
  • Crema S., Cristofolini G., Rossi M., Conte L. High genetic diversity detected in the endemic Primula apennina Widmer (Primulaceae) using ISSR fi ngerprinting // Plant Systematics and Evolution. 2009. Vol. 280. P. 29–36.
  • GBIF: The Global Biodiversity Information Facility. 2023. Available from: https://www.gbif.org. Last accessed 17.04.2023.
  • Guggisberg A., Mansion G., Kelso S., Conti E. Evolution of biogeographic patterns, ploidy levels, and breeding systems in a diploid–polyploid species complex of Primula // New Phytologist. 2006. Vol. 171. N 3. P. 617–632.
  • Hu C.M., Kelso S. Primulaceae // Flora of China. Vol. 15. (Myrsinaceae through Loganiaceae). Beijing, St. Louis. 1996. P. 39–189.
  • Hulten E. Flora of Alaska and Yukon // Acta Universitatis Lundensis. 1948. Vol. 44, N 1. P. 1268.
  • Kovtonyuk N.K., Gontcharov A.A. Phylogenetic relationships in the genus Primula (Primulaceae) inferred from the ITS region sequences of nuclear rDNA // Russian Journal of Genetics. 2009. Vol. 45. N 6. P. 663–670.
  • Martins L., Oberprieler C., Hellwig F. A phylogenetic analysis of Primulaceae s.l. based on internal transcribed spacer (ITS) DNA sequence data // Plant Systematics and Evolution. 2003. Vol. 237. P. 75–85.
  • Mast A.R., Kelso S., Richards A.J., Lang D.J., Feller D.M.S., Conti E. Phylogenetic relationships in Primula L. and related genera (Primulaceae) based on noncoding chloroplast DNA // International Journal of Plant Sciences. 2001. Vol. 162. P. 1381–1400.
  • Nan P., Shi S., Peng S., Tian Ch., Zhong Y. Genetic diversity in Primula obconica (Primulaceae) from Central and South-west China as revealed by ISSR Markers // Annals of Botany. 2003. Vol. 91. P. 329–333.
  • Nei M., Li W.H. Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases // PNAS. 1979. Vol. 76. N 10. P. 5269–5273.
  • Nuzhdina N.S., Kovtonyuk N.K. Genotyping NS and NSK herbaria specimens of Hedysarum (Fabaceae) using DNA sequencing // Botanica Pacifica. A journal of plant science and conservation. 2022. Т. 11. N 1. P. 176–180.
  • POWO. Plants of the World Online. 2023. Facilitated by the Royal Botanic Gardens, Kew. Published on the Internet; http://www.plantsoftheworldonline.org [Retrieved 06 March 2023].
  • Richards J. Primula. Timber Press, Portland, Oregon, USA. 346 p.
  • Schmidt–Lebuhn, A.N., Vos J.M., Keller B., Conti E. 2012. Phylogenetic analysis of Primula section Primula reveals rampant non–monophyly among morphologically distinct species // Molecular Phylogenetics and Evoution. 2012. Vol. 65. N 1. P. 23–34.
  • Tamura K., Stecher G., Ku mar S. MEGA11: Molecular Evolutionary Genetics Analysis Version 11 // Molecular Biology and Evolution. 2001. Vol. 38. N 7. P. 3022–3027.
  • Theodoridis S., Nogues Bravo D., Conti E. The role of cryptic diversity and its environmental correlates in global conservation status assessments: Insights from the threatened bird’s eye primrose (Primula farinosa L.) // Diversity and Distributions. 2019. Vol. 25. P. 1457–1471.
  • Van de Peer Y., De Wachter R. TREECON for Windows: A software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment // Computer Applications in the Biosciences. 1994. Vol. 10. P. 569–570.
  • Wolfe A.D. ISSR techniques for evolutionary biology // Methods of Enzymology. 2005. Vol. 395. P. 134–144.
  • Yan H.-F., Hao G., Hu C.-M., Ge X.-J. DNA barcoding in closely related species: A case study of Primula L. sect. Proliferae Pax (Primulaceae) in China // Journal of Systematics and Evolution. 2011. Vol. 49, N 3. P. 225–236.
  • Yudanova S.S., Plugatar S.A., Klimenko Z.K., Zykova V.K., Rubtsova O.L., Vasilyeva O.Yu. Genetic diversity analysis of Rose varieties from Grandifl ora group based on ISSR molecular markers // Northern Asia Plant Diversity. BIO Web of Conferences. 2021. Vol. 38. 00140. https://doi.org/10.1051/bioconf/20213800140
  • Zhang L.B., Kadereit J.W. Classifi cation of Primula sect. Auricula (Primulaceae) based on two molecular data sets (ITS, AFLPs), morphology and geographical distribution // Botanical Journal of the Linnean Society. 2004. Vol. 146. P. 1–26.

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Bulletin of the MOIP (ed. biol.) It is not included in the Web of Science Core Collection, but is indexed in five Thomson Reuters products on the Web of Science platform — Russian Science Citation Index, BIOSIS Previews, BIOSIS Citation Index, Biological Abstracts, Zoological Record.

The Bulletin of the MOIP (otd. biol.) is one of the journals from the RSCI list, and therefore, according to the Recommendations of the Higher Attestation Commission of 10/26/2022 (item 5), it is equated to the journals of the Higher Attestation Commission List from K1.