Four cultivars of Syringa vulgaris L. of Russian and foreign selection: “Velikaya Pobeda”, “P.P. Konchalovsky”, “Sensation”, “Violetta” were propagated in sterile conditions on Murashige and Skoog nutritive medium with normal and with increased in one and a half macrosalts content and with the addition of hormonal growth regulators N6-(Δ2-Isopentenyl) adenine and thidiazuron (1,5 and 0,1 mg/l correspondingly). It was shown that susceptibility to different types of cytokinins is variety-specific. Application of thidiazuron during “Sensation” propagation leads to decreasing of coefficient of propagation, and for three other cultivars – to it`s increasing. Time of maintaining in sterile culture and the number of passages practically don`t have influence over regenerative abilities of lilac cv. “Violetta”.
2019. T. 124. Vyp. 5.
2019. T. 124. Vyp. 5.
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The Influence of Nutritive Medium Composition and Thidiazuron on Realization of Morphogenetic Potential in vitro of Different Lilac Cultivars
References
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Список литературы
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- Набиева А.Ю. Биотехнологические приемы клонального микроразмножения перспективных сортов Syringa vulgaris L. для Западной Сибири // Вестн. ИрГСХА. 2011. Т. 5. № 44. С. 69–76 [Nabieva A.Yu. Biotekhnologicheskie priemy klonal’nogo mikrorazmnozheniya perspektivnykh sortov Syringa vulgaris L. dlya Zapadnoi Sibiri // Vestn. IrGSKhA. 2011. T. 5. № 44. S. 69–76].
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- Arndt F.R., Rusch R., Stillfried H.V., Hanisch B., Martin W.C. A new cotton defoliant // Plant Physiol. 1976. Vol. 57. P. 99.
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- Churikova O.A. Microclonal propagation and some regularities of morphogenesis of daylily and hosta in vitro // Moscow Univ. Biol. Sci. Bull. 2008. Vol. 63, N 2. P. 89–94.
- Einset J.W., Alexander J.H. Multiplication of Syringa species and cultivars in tissue culture // Comb. Proc. Intern. Plant Propagators Soc. 1985. Vol. 34. P. 628–636.
- Faisal M., Anis M. Thidiazuron induced high frequency axillary shoot multiplication in Psoralea corylifolia // Biologia Plantarum. 2006. Vol. 50. N 3. P. 437–440.
- Favre J.M., Juncker B. In vitro growth of buds taken from seedlings and adult plant material in Quercus robur L. // Plant Cell, Tissue and Organ Culture. 1987. Vol. 8. P. 49–60.
- Gabryszewska E. Effect of various levels of sucrose, nitrogen salts and temperature on the growth and development of Syringa vulgaris L. shoots in vitro // Journal of Fruit and Ornamental Plant Research. 2011. Vol. 19. N 2. P. 113–148.
- Guo B., Abbasi B.H., Zeb A., Xu L.L., Wei Y.H. Thidiazuron: A multi-dimensional plant growth regulator // African Journal of Biotechnology. 2011. Vol. 10. N 45. P. 8984–9000.
- Hand C., Maki S., Reed B.M. Modeling optimal mineral nutrition for hazelnut micropropagation // Plant Cell, Tissue and Organ Culture. 2014. Vol. 119. P. 411–425.
- Huetteman C.A., Preece J.E. Thidiazuron: a potent cytokinin for woody plant tissue culture // Plant Cell, Tissue and Organ Culture. 1993. Vol. 33. P. 105–119.
- Jabbarzadeh Z., Khosh-Khui M. Factors affecting tissue culture of Damask rose (Rosa damascena Mill.) // Scientia Horticulturae. 2005. Vol. 105. P. 475–482.
- Jimenez V., Castillo J., Tavare E., Guevara E., Montie M. In vitro propagation of the neotropical giant bamboo, Guadua angustifolia Kunth, through axillary shoot proliferation // Plant Cell, Tissue and Organ Culture. 2006. Vol. 86. P. 389–395.
- Kamenski P.A., Sazonov A.E., Fedyanin A.A., Sadovnichy V.A. Biological collections: Chasing the ideal // Acta Naturae. 2016. Vol. 8. P. 6–9.
- Mashkina O.S., Tabatskaya T.M., Starodubtseva L.M. Mass clonal propagation of karelian birch and poplar through long-term shoot multiplication // Russian Journal of Plant Physiology. 1999. Vol. 46, N 6. P. 835–837.
- Molkanova O.I., Spirydovich E.V., Konovalova L.N., Brel N.G., Zinina U.M., Reshetnikov V.N. Complex observation of introduction species and varieties of Syringa L. in Main Botanical Garden of the RAS and in the Central Botanical Garden of the NAS of Belarus // Bulletin of Udmurt University. Biology and Earth Sciences. 2011. Vol. 2. P. 66–73.
- Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures // Physiol. Plant. 1962. Vol. 15. P. 473–497.
- Nesterowicz S., Kulpa D., Moder K., Kurek J. Micropropagation of an old specimen of common lilac (Syringa vulgaris L.) from the dendrological garden at Przelewice // Hortorum Cultus. 2006. Vol. 5. N 1. P. 27–35.
- Norton M.E., Norton C.R. Change in shoot proliferation with repeated in vitro subculture of shoots of woody species of Rosaceae // Plant Cell, Tissue and Organ Culture. 1986. Vol. 5. P. 187–197.
- Pierik R.L.M., Steegmans H.H.M., Elias A.A., Stiekema O.T.J., van der Velde A.J. Vegetative propagation of Syringa vulgaris L. in vitro // Acta Horticulturae. 1988. Vol. 226. P. 195–204.
- Ramanayake S.M.S.D., Yakandawala K. Micropropagation of the giant bamboo (Dendrocalamus giganteus Munro) from nodal explants of field grown culms // Plant Sci. 1997. Vol. 129. P. 213–223.
- Sharma K.K., Thorpe T.A. In vitro propagation of mulberry (Morus alba L.) through nodal segments // Scientia Horticulturae. 1990. Vol. 42. N 4. P. 307–320.
- Tomsone S., Galeniece Ā., Akere A., Priede G., Zīra L. In vitro propagation of Syringa vulgaris L. cultivars // Biologija. 2007. Vol. 53. Vol. 2. P. 28–31.
- Van Nieuwkerk J.P., Zimmerman R.H., Fordham I. Thidiazuron stimulation of apple shoot proliferation in vitro // Hort. Sci. 1986. Vol. 21. P. 516–518.